Analysis of Functional Components of High Performance Liquid Chromatograph

High performance liquid chromatograph is a frequently used instrument and equipment in the experiment, qualitative and quantitative analysis of substances, especially in the analysis of environmental pollution has an important application. However, many people are not very clear about the structure of HPLC. The following is a brief introduction.

High performance liquid chromatography is generally composed of reservoirs, pumps, injectors, columns, detectors, and recorders.

1, reservoir. Mainly used to store the mobile phase of the sample solution.

2, high pressure pump. Also known as high-pressure infusion pumps, the sample and the mobile phase are mainly used to enter the chromatographic separation column and detection system.

3, sampler. It is a device that the sample solution must enter into the mobile phase. It mainly introduces the sample to be analyzed into the chromatographic system. Syringes, valve injections, stop injections, and autosamplers are common types of injectors.

4, the column. Mainly by the column tube, pressure cap, card sleeve (sealing ring), sieve plate (filter), joints, screws and other components for the separation of the mixture.

5, detector. It is mainly used to analyze the sample concentration, convert the concentration into electrical signals, and transmit them.

6, recorder. It is mainly used to receive the electrical signal transmitted by the detector, and finally prints the data in the form of a graph.